In an attempt to decrease false positives, a 1014 day waiting period was initiated to allow inflammatory lesions to resolve before application of stain. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components. Toluidine blue stain for mast cells urate crystal stain vonkossa stain for calcium. Toluidine blue, 1% aqueous safety data sheet according to federal register vol. It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. Cut ultrathin sections 60 nm thick with ultramicrotome.
Toluidine blue 1% aqueous stain solution other means of identification. Toluidine blue tb is a polychromatic dye which can absorb different colours depending on how it binds chemically with various tissue components. Although toluidine blue o is used routinely as a stain in animal cytol ogy, and. Stain sections with 1% toluidine blue stain and choose region containing many spheroplasts. Hazard identification emergency overview appearance. An easily prepared sulfation reagent made with sulfuric and acetic acids was used. Toluidinebluefast greenstain basicinfotoluidineblueisa. Toluidine blue, 1% biological stain lab chemical 15ml. Blue is the complementary colour of yellow and with red and green forms a set of primary colours 2. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database.
Basic blue 17, blutene chloride, methylene blue t50 or t extra, tolonium chloride cas number 92319. Rinse the sample by adding 12 drops of water and then soaking up the liquid again. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for acetoorcein stain results. Pneumocystis carinii is an opportunist which causes a diffuse interstitial pneumonia in patients with impaired immune systems. The stain can be employed for bronchoalveolar lavages and lung tissue touch preparations. Toluidine blue definition of toluidine blue by medical. Toluidine blue o certified by the biological stain. Composition information on ingredients principle hazardous components chemical and common names regulated cas. Tolonium chloride, basic blue 17 company name ted pella, inc. Blue or violet dyes will show a red color shift, and red dyes will. Safety data sheet creation date 11mar2010 revision date 18jan2018 revision number 4 1. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in dna and rna. Pdf toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic.
It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property. A modified toluidine blue o staining technique for pneumocystis carinii is described. Composition 1 % toluidine blue in 50 % isopropanol. Place copper grids in acetone for 5 min and dry on filter paper. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number. Toluidine blue stain definition of toluidine blue stain. Pneumocystis carinii stain kit modified toluidine blue o. Both safranin o and toluidine blue are cationic dyes that stain proteoglycans as well as glycosaminoglycans. Solution i toluidine blue o stain solution 11 95% ethyl alcohol solution iii absolute alcohol solution iv xylene. Stain sections in toluidine blue working solution for 23 minutes.
To do this staining, after air drying of smears, they were fixed in fresh 96% ethanolacetone 1. Add 12 drops toluidine blue and let sit for about 30 seconds to 1 minute. In vivo, toluidine blue stains deoxyribonucleic acid andor. Fites acid fast stain alcian blue stain alcian blue pas stain pab hyaluronidase digestion for alcian blue alizarin stain for calcium auraminerhodamine stain fluorescent bielschowsky stain for senile plaques bile stain bodians stain colloidal iron stain congo red stain copper stain elastic van gieson stain elastic weigerts resorcin. It is a member of the thiazine group and is partially. Toluidine blue o 36412 25g glass bottle application certified by the biological stain commission bsc for invitro diagnostic use. Toluidine blue is a biological stain for staining nuclear material during mitosis. Division of radiooncology, deutsches krebsforschungszentrum, 69120 heidelberg, germany subsequent to immunohistological reaction, counterstaining of tissue sections with the thiazin dye toluidine blue toluidine blue o is an alternative to other dyes e. Jan 28, 2014 toluidine blue tb is a polychromatic dye which can absorb different colours depending on how it binds chemically with various tissue components. Harleco for use in histological sections, animal tissue and as a general nuclear stain. One can also see the entire issue as printed here in a flip book version on desktops. An evaluation of novel vital dyes for intraocular surgery. It is used as a medical aid to selectively stain tissues in a patient undergoing eye surgery. Tolonium chloride certified biological stain recommended use laboratory chemicals.
Fluorescent stain the patient uses a special fluorescent mouth rinse and then a special light is used to look around in the. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components it stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. Some protocol mention dissolving toluidine blue in distilled water, some in ethanol and some in acetate buffer. Toluidine blue table ii has been reported to provide more intense staining, due to the fact that it has a higher affinity for the sulfur in cartilage compared to safranin o 3 table iii. Toluidine blue synonyms, toluidine blue pronunciation, toluidine blue translation, english dictionary definition of toluidine blue.
Metachromasia, tissue elements staining a different color from the dye solution, is due to the ph, dye concentration and temperature of the basic dye. Moreover, the staining procedure is rapid and easily performed, thus, toluidine blue is helpful and widely employed for any kind of overview stainings. Staining is widely used in histopathology and diagnosis, as it allows for the identification of abnormalities in cell count and structure under the microscope. Toluidine blue is also commonly used to stain frozen sections rapid microscopic analysis of a specimen. Toluidine blue staining as an adjunctive tool for early. Allow the smear to air dry and examine microscopically. Material safety data sheet toluidine blue, 1% aqueous. When evaluated as a part of the clinical examination, toluidine blue staining may provide additional information. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in dnaand rna. Toluidine blue is a cationic metachromatic dye that may selectively bind to free anionic groups such as sulfate, phosphate, and carboxylate radicals of large molecules 5, 34. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for acetoorcein stain. Jan 06, 2017 colour of the dye changes from blue to purplered. Clear in xylene or xylene substitute, 2 changes, 3 minutes each. Toluidine blue staining staining protocol sample type required.
Toluidine blue table ii has been reported to provide more intense staining, due to the fact that it has a higher affinity for the sulfur in cartilage. Spray the target area with 1% n acetylcysteine solution. Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Section 1 chemical product and company identification. Toluidine blue stain a procedure in which a blue dye is placed on any lesions found in the mouth. Toluidine blue stains esophageal malignancies and dysplasia, although lugols iodine solution has supplanted it to a great extent. Toluidine blue staining for mast cells linkedin slideshare. The manuals for this section have been compiled by susan l. The composition of this mountant is modified from that proposed by.
Packaging 5, 25 g in glass bottle biochemphysiol actions toluidine blue o tbo is a thiazine dye of the quinoneimine family and is cationic in nature. Toluidine blue staining is considered to be sensitive in identifying early oropharyngeal premalignant and malignant lesions. Toluidine blue definition of toluidine blue by merriam. For use in histological sections, animal tisuue and as a general nuclear stain. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage. Toluidine blue staining of resinembedded sections for. Can the use of vital staining limit the number of routine biopsies for. Toluidine blue o along with lightemitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gramnegative bacteria.
What is best preparation for toluidine blue reagent for. Toluidine blue is often used to identify mast cells, by virtue of the heparin in their cytoplasmic granules. Polychromatic staining of plant cell walls by toluidine blue o. Basic methods in histopathology of joint tissues sciencedirect. Identification product name toluidine blue o cat no. Page 15 safety data sheet according to safe work australia printing date 12. Alcian blue stain alcian bluepas stain pab hyaluronidase digestion for alcian blue. This article provides an overview on chemistry, technique, and the various applications of toluidine blue. Advantages of a modified toluidine blue o stain and. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Trypan blue uses, side effects, substitutes, composition. Toluidine blue color perception in identification of oral.
Sigmaaldrich material safety data sheet product name. Toluidine blue article about toluidine blue by the free. As it is absorbed by nucleic acids, toluidine blue stains malignant or dysplastic lesions with elevated mitotic activity. Pneumocystis carinii stain kit modified toluidine blue o stain. It is usually administered as an injection into the anterior chamber of the eyes.
Product identifier and chemical identity product name. Prepare 1% solution of toluidine blue by dissolving 2 g of sodium borate in 100 ml of deionized water, then add 1 g of toluidine blue and stir until dissolved. Corneybacterium diphtheria contains granules with polymerized inorganic polyphosphate, which stains red violet color. Toluidine blue o certified by the biological stain commission. Toluidine blue should stain mast cells redpurple metachromatic staining and the background blue orthochromatic staining. Toluidine blue staining protocol for mast cells ihc world. At the present time, the only clinical method of detecting these foci of. In the present study, the use of toluidine blue staining was taken into consideration to identify clinically doubtful oropharyngeal lesions and to compare toluidine blue stain and with the histological evaluation. The intensity of dark blue staining seen with the 0.
It is also used to stain proteoglycans and glycosaminoglycans in tissues such as cartilage. Differentiate the sections in absolute isopropanol till clear background. Uses advised against not for food, drug, pesticide or biocidal. Microscopic examination of urine sediment flashcards quizlet. Apr 23, 2020 staining is widely used in histopathology and diagnosis, as it allows for the identification of abnormalities in cell count and structure under the microscope. Any areas that appear darker once the blue stain is added may be cancerous and will need to be examined further. Toluidine blue table ii has been reported to provide more intense staining, due to the fact that it has a higher affinity for the sulfur in. Dehydrate quickly through 95% and 2 changes of 100% alcohol 10 dips each since stain fades quickly in alcohol. Basic blue 17 dimethyltoluthionine chloride f klot gabilin klot menodin modr toluidinova czech modr zasadita 17 czech schultz 1041 tolazul tolonium chloride toluidinblau german toluidine blue toluidine blue o toluidine blue oo toluidine blue phenothiazine dye. Toluidine blue definition is a basic thiazine dye that is related to methylene blue and is used as a biological stain. Certain stains change the coloration of cells and tissues significantly, different from the color. The strongly acidic macromolecular carbohydrates of mast cells and cartilage are coloured red by the blue dye, a phenomenon called metachromasia. Use a tissue to absorb the toluidine blue by soaking up from edge of liquid.
Helicobacter stains dark blue against a variably blue background. Toluidine blue stain definition of toluidine blue stain by. Safranin o and toluidine blue both safranin o and toluidine blue are cationic dyes that stain proteoglycans as well as glycosaminoglycans. Toluidine blue stain an overview sciencedirect topics. Toluidine blue o certified by the biological stain commission synonym. It first emerged in 1856, courtesy of a british chemist called william henry perkin. It stains nucleic acids blue and polysaccharides purple and. Procedure and uses august 1, 2014 acharya tankeshwar bacteriology, staining techniques in microbiology 0 bacteria are easier to detect in broth culture if the preparation is stained using toluidine blue 0.
Itsstainingpropertiesare dramaticallyaltereddependingonthefollowingparameters. A huge range of stains is used in histology, from dyes and metals to labeled antibodies. The toluidine blue stains the neural cytoplasm of the ganglion, allowing for easy identification of these cells. Toluidine blue definition of toluidine blue by the free. Toluidine blue is the agent most commonly used for vital staining of the mucosa of the. Toluidine blue tb has an affinity for nucleic acids, and therefore binds to nuclear material of tissues with a high dna and rna content.